List of Contributors ............................................ v
Preface ....................................................... vii
Flow techniques in NMR spectroscopy
P.A.Keifer
1. Introduction ................................................. 2
2. The start of flow NMR and the evolution of LC-NMR ............ 3
3. Non-chromatographic flow NMR ................................ 13
4. New technologies to facilitate flow NMR ..................... 21
5. Variations of the flow techniques/fluidics .................. 30
6. Applications ................................................ 33
7. Problems and challenges ..................................... 36
8. Conclusions ................................................. 40
Acknowledgments ................................................ 40
References ..................................................... 40
Vanadium-51 NMR
D.Rehder, T.Polenova and M.Buhl
1. Introduction ................................................ 50
2. Isotropic systems ........................................... 51
3. Solid-state parameters ...................................... 81
4. Mesophases .................................................. 94
5. Computational methods ....................................... 97
6. Concluding remarks ......................................... 106
Acknowledgments ............................................... 107
References .................................................... 107
Solid-State Effects on NMR Chemical Shifts
A.M.Orendt and J.С.Facelli
1. Introduction ............................................... 116
2. Origin of solid-state effects on chemical shifts ........... 119
3. Theoretical methods ........................................ 126
4. Case studies of solid-state effects ........................ 134
5. Concluding remarks ......................................... 173
Acknowledgments ............................................... 174
References .................................................... 174
NMR Studies of Encapsulated Macromolecules
P.F.Flynn, A.K.Simorellis and W.D.Van Horn
1. Introduction ............................................... 180
2. The challenge of complex biomacromolecules ................. 181
3. The rate of NMR relaxation depends on the molecular
tumbling rate .............................................. 182
4. Reverse micelles ........................................... 185
5. Water in reverse micelles .................................. 188
6. Thermodynamics of reverse micelle particle formation ....... 189
7. Proteins encapsulated by reverse micelles .................. 190
8. Encapsulation protocols .................................... 191
9. Solvent suppression ........................................ 192
10.Hydrodynamic behavior of AOT reverse micelle assemblies .... 193
11.Practical application of reverse micelle encapsulation to
larger polypeptides ........................................ 197
12.Multinuclear resonance assignments of encapsulated
proteins ................................................... 198
13.Structural studies of encapsulated macromolecules .......... 199
14.Low temperature NMR studies of encapsulated water .......... 201
15.Thermodynamics of water shedding - a concise analysis ...... 202
16.Low temperature studies of encapsulated proteins ........... 205
17.Effect of reverse micelle encapsulation on motional
dynamics in polypeptides ................................... 211
18.Future studies ............................................. 214
Acknowledgments ............................................... 215
References .................................................... 215
Index ......................................................... 221
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