Sauer M. Handbook of fluorescence spectroscopy and imaging: from single molecules to ensembles (Weinheim, 2011). - ОГЛАВЛЕНИЕ / CONTENTS
Навигация

Архив выставки новых поступлений | Отечественные поступления | Иностранные поступления | Сиглы
ОбложкаSauer M. Handbook of fluorescence spectroscopy and imaging: from single molecules to ensembles / M.Sauer, J.Hofkens, J.Enderlein. - Weinheim: Wiley-VCH, 2011. - ix, 281 p. - Incl. bibl. ref. - Ind.: p.273-281. - ISBN 978-3-527-31669-4
 

Оглавление / Contents
 
   Preface ..................................................... IX
   
1  Basic Principles of Fluorescence Spectroscopy ................ 1
   1.1  Absorption and Emission of Light ........................ 1
   1.2  Spectroscopic Transition Strengths ...................... 5
   1.3  Lambert-Beer Law and Absorption Spectroscopy ............ 7
   1.4  Fluorophore Dimerization and Isosbestic Points .......... 9
   1.5  Franck-Condon Principle ................................ 12
   1.6  Temperature Effects on Absorption and Emission 
        Spectra ................................................ 15
   1.7  Fluorescence and Competing Processes ................... 17
   1.8  Stokes Shift, Solvent Relaxation, and Solvatochroism ... 20
   1.9  Fluorescence Quantum Yield and Lifetime ................ 22
   1.10 Fluorescence Anisotropy ................................ 27
   References .................................................. 29
2  Fluorophores and Fluorescent Labels ......................... 31
   2.1  Natural Fluorophores ................................... 31
   2.2  Organic Fluorophores ................................... 35
   2.3  Different Fluorophore Classes .......................... 38
   2.4  Multichromophoric Labels ............................... 49
   2.5  Nanocrystals ........................................... 52
   References .................................................. 56
3  Fluorophore Labeling for Single-Molecule Fluorescence
   Spectroscopy (SMFS) ......................................... 61
   3.1  In Vitro Fluorescence Labeling ......................... 61
   3.2  Fluorescence Labeling in Living Cells .................. 69
   References .................................................. 80
4  Fluorophore Selection for Single-Molecule Fluorescence 
   Spectroscopy (SMFS) and Photobleaching Pathways ............. 85
   References .................................................. 91
5  Fluorescence Correlation Spectroscopy ....................... 93
   5.1  Introduction ........................................... 93
   5.2  Optical Set-Up ......................................... 98
   5.3  Data Acquisition and Evaluation ........................ 99
   5.4  Milliseconds to Seconds: Diffusion and 
        Concentration ......................................... 103
   5.5  Nanoseconds to Microseconds: Photophysics,
        Conformational Fluctuations, Binding Dynamics ......... 120
   5.6  Picoseconds to Nanoseconds: Rotational Diffusion and
        Fluorescence Antibunching ............................. 122
   5.7  Fluorescence Lifetime Correlation Spectroscopy ........ 135
   5.8  Conclusion ............................................ 143
   References ................................................. 143
6  Excited State Energy Transfer .............................. 147
   6.1  Introduction .......................................... 147
   6.2  Theory of (Forster) Energy Transfer ................... 148
   6.3  Experimental Approach for Single-Pair FRET-
        Experiments ........................................... 157
   6.4  Examples and Applications of FRET ..................... 161
   References ................................................. 183
7  Photoinduced Electron Transfer (PET) Reactions ............. 189
   7.1  Fluorescence Quenching by PET ......................... 189
   7.2  Single-Molecule Fluorescence Spectroscopy to Study 
        PET ................................................... 192
   7.3  Single-Molecule Sensitive Fluorescence Sensors Based
        on PET ................................................ 199
   7.4  PET Reporter System ................................... 202
   7.5  Monitoring Conformational Dynamics and Protein 
        Folding by PET ........................................ 205
   7.6  Biological and Diagnostic Applications ................ 209
   References ................................................. 215
8  Super-Resolution Fluorescence Imaging ...................... 219
   8.1  Diffraction Barrier of Optical Microscopy ............. 219
   8.2  Multi-Photon and Structured Illumination Microscopy ... 221
   8.3  Stimulated Emission Depletion ......................... 223
   8.4  Single-Molecule Based Photoswitching Microscopy ....... 226
   8.5  Background and Principles of Single-Molecule Based
        Photoswitching Microscopy Methods ..................... 229
   8.6  Temporal Resolution of Super-Resolution Imaging 
        Methods ............................................... 236
   References ................................................. 237
9  Single-Molecule Enzymatics ................................. 241
   9.1  Introduction: Why Study Enzymes on a Single-Molecule
        Level? ................................................ 241
   9.2  Biochemical Principles of Enzymatic Activity: 
        the Michaelis-Menten Model ............................ 242
   9.3  "Looking" at Individual Enzymes ....................... 243
   
   9.4  Data Analysis of Fluorescence Intensity Time Traces
        of Single-Turnover Experiments ........................ 264
   9.5  Conclusions ........................................... 267
   References ................................................. 268

   Index ...................................................... 273


Архив выставки новых поступлений | Отечественные поступления | Иностранные поступления | Сиглы
 

[О библиотеке | Академгородок | Новости | Выставки | Ресурсы | Библиография | Партнеры | ИнфоЛоция | Поиск]
  Пожелания и письма: branch@gpntbsib.ru
© 1997-2024 Отделение ГПНТБ СО РАН (Новосибирск)
Статистика доступов: архив | текущая статистика
 

Документ изменен: Wed Feb 27 14:23:10 2019. Размер: 9,207 bytes.
Посещение N 1460 c 14.02.2012