MAP kinase signaling protocols / ed. by R.Seger. - 2nd ed. - New York: Humana Press, 2010. - xiv, 529 p.: ill. (some col.). - (Springer protocols. Methods in molecular biology; 661). - Incl. bibl. ref. - Ind.: p.525-529. - ISSN 1064-3745; ISBN 978-1-60761-794-5  Место хранения:   056 | Институт химической биологии и фундаментальной медицины CO РАН | Новосибирск| Библиотека

Оглавление / Contents

Preface ......................................................... v Contributors ................................................... xi Part I Activation and Function of Components of the MAP Kinase Signaling Cascades 1 The MAP Kinase Signaling Cascades: A System of Hundreds of Components Regulates a Diverse Array of Physiological Functions .................................................... 3 Yonat Keshet and Rony Seger 2 Determination of ERK Activity: Anti-phospho-ERK Antibodies and In Vitro Phosphorylation ................................ 39 Shiri Procaccia, Sarah Kraus, and Rony Seger 3 Activation of SAPK/JNKs In Vitro ............................ 59 Deborah N. Chadee and John M. Kyriakis 4 Activation of p38 and Determination of Its Activity ......... 75 Huatnin Zhou, Jianming Chen, and Jiahuai Han 5 Activity Assays for Extracellular Signal-Regulated Kinase 5 .................................................... 91 Kazuhiro Nakamura and Gary L. Johnson 6 Use of Inhibitors in the Study of MAP Kinases .............. 107 Kimberly Burkhard and Paul Shapiro Part II Study of MAP Kinase Cascades as Transmitters of Membranal Receptor Signals 7 MAP Kinase Activation by Receptor Tyrosine Kinases: In Control of Cell Migration ............................... 125 Gabi Tarcic and Yosef Yarden 8 Activation of Ras and Rho GTPases and MAP Kinases by G-Protein-Coupled Receptors ............................. 137 Mario Chiariello, Jose P. Vaqué, Piero Crespo, and J. Silvio Gutkind 9 Regulation of MAP Kinase Signaling by Calcium .............. 151 Colin D. White and David B. Sacks 10 Identification of Novel Substrates of MAP Kinase Cascades Using Bioengineered Kinases that Uniquely Utilize Analogs of ATP to Phosphorylate Substrates ......................... 167 Hui Zheng, Adnan Al-Ayoubi, and Scott T. Eblen 11 ERK-MAP Kinase Signaling in the Cytoplasm .................. 185 Michelle C. Mendoza, Ekrem Emrah Er, and John Blenis 12 Lentiviral Vectors to Study the Differential Function of ERK1 and ERK2 MAP Kinases .................................. 205 Marzia Indrigo, Alessand.ro Papale, Daniel Orellana, and Riccardo Brambilla Part III Structure-Function Relationships and Localization of MAP Kinases 13 Structural Studies of MAP Kinase Cascade Components ........ 223 Elizabeth J. Goldsmith, Xiaoshan Min, Haixia He, and Tianjun Zhou 14 Analysis of MAP Kinases by Hydrogen Exchange Mass Spectrometry ............................................... 239 Kevin M. Sours and Natalie G. Ahn 15 A "Molecular Evolution" Approach for Isolation of Intrinsically Active (MEK-Independent) MAP Kinases ......... 257 Vered Levin-Salomon, Oded Livnah, and David Engelberg 16 Reconstitution of the Nuclear Transport of the MAP Kinase ERK2 ...................................................... 273 Arifjivan, Aarati Ranganatban, and Melanie H. Cobb 17 Localization and Trafficking of Fluorescently Tagged ERK1 and ERK2 ................................................... 287 Matilde Marchi, Riccardo Parra, Mario Costa, and Gian Michele Ratto Part IV Studies on the Regulation of MAP Kinase Cascades 18 Studying the Regulation of MAP Kinase by MAP Kinase Phosphatases In Vitro and in Cell Systems .................. 305 Céline Tárrega, Caroline Nunes-Xavier, Rocío Cejudo-Marín, Jorge Martin-Pérez, and Rafael Pulido 19 Proteomic Analysis of Scaffold Proteins in the ERK Cascade .................................................... 323 Melissa M. McKay and Deborah K. Morrison 20 Analysis of ERKs' Dimerization by Electrophoresis .......... 335 Adán Pinto and Piero Crespo 21 MAP Kinase: SUMO Pathway Interactions ...................... 343 Shen-Hsi Yang and Andrew D. Sharrocks 22 Computational Modelling of Kinase Signalling Cascades ...... 369 David Gilbert, Monika Heiner, Rainer Breitling, and Richard Orton Part V Use of Lower Organisms, Animal Models, and Human Genetics in the Study of MAP Kinases 23 Analysis of Mitogen-Activated Protein Kinase Activity in Yeast ...................................................... 387 Elaine A. Elion and Rupam Sahoo 24 Detection of RTK Pathway Activation in Drosophila Using Anti-dpERK Immunofluorescence Staining ..................... 401 Aharon Helman and Ze'ev Paroush 25 Studying MAP Kinase Pathways During Early Development of Xenopus laevis ............................................. 409 Aviad Keren and Eyal Bengal 26 Deciphering Signaling Pathways In Vivo: The Ras/Raf/Mek/Erk Cascade .................................... 421 Gergana Galabova-Kovacs and Manuela Baccarini 27 Mutational and Functional Analysis in Human Ras/MAP Kinase Genetic Syndromes .......................................... 433 William E. Tidyman and Katherine A. Rauen Part VI Study of MAP Kinases in Specific Physiological Systems 28 Implication of the ERK Pathway on the Post-transcriptional Regulation of VEGF mRNA Stability .......................... 451 Khadija Essafi-Benkhadir, Jacques Pouysségur, and Gilles Pagès 29 Studies on MAP Kinase Signaling in the Immune System ....... 471 Hongbo C.M. and Richard A. Flavell 30 Methods to Study MAP Kinase Signalling in the Central Nervous System ............................................. 481 Bettina Wagner and Maria Sibilia 31 MAP Kinase Regulation of the Mitotic Spindle Checkpoint .... 497 Eva M. Eves and Marsha Rich Rosner 32 Using High-Content Microscopy to Study Gonadotropin Releasing Hormone Regulation of ERK ........................ 507 Christopher J. Caunt, Stephen P. Armstrong, and Craig A. McArdle Index ......................................................... 525