MAP kinase signaling protocols (New York, 2010). - ОГЛАВЛЕНИЕ / CONTENTS
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ОбложкаMAP kinase signaling protocols / ed. by R.Seger. - 2nd ed. - New York: Humana Press, 2010. - xiv, 529 p.: ill. (some col.). - (Springer protocols. Methods in molecular biology; 661). - Incl. bibl. ref. - Ind.: p.525-529. - ISSN 1064-3745; ISBN 978-1-60761-794-5
 

Оглавление / Contents
 
Preface ......................................................... v
Contributors ................................................... xi

Part I    Activation and Function of Components of the MAP
          Kinase Signaling Cascades

1  The MAP Kinase Signaling Cascades: A System of Hundreds
   of Components Regulates a Diverse Array of Physiological
   Functions .................................................... 3
   Yonat Keshet and Rony Seger
2  Determination of ERK Activity: Anti-phospho-ERK Antibodies
   and In Vitro Phosphorylation ................................ 39
   Shiri Procaccia, Sarah Kraus, and Rony Seger
3  Activation of SAPK/JNKs In Vitro ............................ 59
   Deborah N. Chadee and John M. Kyriakis
4  Activation of p38 and Determination of Its Activity ......... 75
   Huatnin Zhou, Jianming Chen, and Jiahuai Han
5  Activity Assays for Extracellular Signal-Regulated
   Kinase 5 .................................................... 91
   Kazuhiro Nakamura and Gary L. Johnson
6  Use of Inhibitors in the Study of MAP Kinases .............. 107
   Kimberly Burkhard and Paul Shapiro

Part II   Study of MAP Kinase Cascades as Transmitters of
          Membranal Receptor Signals
7  MAP Kinase Activation by Receptor Tyrosine Kinases:
   In Control of Cell Migration ............................... 125
   Gabi Tarcic and Yosef Yarden
8  Activation of Ras and Rho GTPases and MAP Kinases
   by G-Protein-Coupled Receptors ............................. 137
   Mario Chiariello, Jose P. Vaqué, Piero Crespo, and
   J. Silvio Gutkind
9  Regulation of MAP Kinase Signaling by Calcium .............. 151
   Colin D. White and David B. Sacks
10 Identification of Novel Substrates of MAP Kinase Cascades
   Using Bioengineered Kinases that Uniquely Utilize Analogs
   of ATP to Phosphorylate Substrates ......................... 167
   Hui Zheng, Adnan Al-Ayoubi, and Scott T. Eblen
11 ERK-MAP Kinase Signaling in the Cytoplasm .................. 185
   Michelle C. Mendoza, Ekrem Emrah Er, and John Blenis
12 Lentiviral Vectors to Study the Differential Function of
   ERK1 and ERK2 MAP Kinases .................................. 205
   Marzia Indrigo, Alessand.ro Papale, Daniel Orellana, and
   Riccardo Brambilla

Part III Structure-Function Relationships and Localization
         of MAP Kinases

13 Structural Studies of MAP Kinase Cascade Components ........ 223
   Elizabeth J. Goldsmith, Xiaoshan Min, Haixia He, and
   Tianjun Zhou
14 Analysis of MAP Kinases by Hydrogen Exchange Mass
   Spectrometry ............................................... 239
   Kevin M. Sours and Natalie G. Ahn
15 A "Molecular Evolution" Approach for Isolation of
   Intrinsically Active (MEK-Independent) MAP Kinases ......... 257
   Vered Levin-Salomon, Oded Livnah, and David Engelberg
16 Reconstitution of the Nuclear Transport of the MAP Kinase
    ERK2 ...................................................... 273
   Arifjivan, Aarati Ranganatban, and Melanie H. Cobb
17 Localization and Trafficking of Fluorescently Tagged ERK1
   and ERK2 ................................................... 287
   Matilde Marchi, Riccardo Parra, Mario Costa, and Gian
   Michele Ratto

Part IV  Studies on the Regulation of MAP Kinase Cascades

18 Studying the Regulation of MAP Kinase by MAP Kinase
   Phosphatases In Vitro and in Cell Systems .................. 305
   Céline Tárrega, Caroline Nunes-Xavier, Rocío Cejudo-Marín,
   Jorge Martin-Pérez, and Rafael Pulido
19 Proteomic Analysis of Scaffold Proteins in the ERK
   Cascade .................................................... 323
   Melissa M. McKay and Deborah K. Morrison
20 Analysis of ERKs' Dimerization by Electrophoresis .......... 335
   Adán Pinto and Piero Crespo
21 MAP Kinase: SUMO Pathway Interactions ...................... 343
   Shen-Hsi Yang and Andrew D. Sharrocks
22 Computational Modelling of Kinase Signalling Cascades ...... 369
   David Gilbert, Monika Heiner, Rainer Breitling, and
   Richard Orton

Part V   Use of Lower Organisms, Animal Models, and Human
         Genetics in the Study of MAP Kinases

23 Analysis of Mitogen-Activated Protein Kinase Activity in
   Yeast ...................................................... 387
   Elaine A. Elion and Rupam Sahoo
24 Detection of RTK Pathway Activation in Drosophila Using
   Anti-dpERK Immunofluorescence Staining ..................... 401
   Aharon Helman and Ze'ev Paroush
25 Studying MAP Kinase Pathways During Early Development of
   Xenopus laevis ............................................. 409
   Aviad Keren and Eyal Bengal
26 Deciphering Signaling Pathways In Vivo: The
   Ras/Raf/Mek/Erk Cascade .................................... 421
   Gergana Galabova-Kovacs and Manuela Baccarini
27 Mutational and Functional Analysis in Human Ras/MAP Kinase
   Genetic Syndromes .......................................... 433
   William E. Tidyman and Katherine A. Rauen

Part VI  Study of MAP Kinases in Specific Physiological Systems

28 Implication of the ERK Pathway on the Post-transcriptional
   Regulation of VEGF mRNA Stability .......................... 451
   Khadija Essafi-Benkhadir, Jacques Pouysségur, and Gilles
   Pagès
29 Studies on MAP Kinase Signaling in the Immune System ....... 471
   Hongbo C.M. and Richard A. Flavell
30 Methods to Study MAP Kinase Signalling in the Central
   Nervous System ............................................. 481
   Bettina Wagner and Maria Sibilia
31 MAP Kinase Regulation of the Mitotic Spindle Checkpoint .... 497
   Eva M. Eves and Marsha Rich Rosner
32 Using High-Content Microscopy to Study Gonadotropin
   Releasing Hormone Regulation of ERK ........................ 507
   Christopher J. Caunt, Stephen P. Armstrong, and Craig
   A. McArdle

Index ......................................................... 525


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