Preface ......................................................... v
Contributors ................................................... xi
1 Utilization of Liposomes for Studying Drug Transfer and
Uptake ....................................................... 1
Alfred Fahr and Xiangli Liu
2 The Use of Liposomes in the Study of Drug Metabolism:
A Method to Incorporate the Enzymes of the Cytochrome P450
Monooxygenase System into Phospholipid, Bilayer Vesicles .... 11
James R. Reed
3 Use of Liposomes to Study Cellular Osmosensors .............. 21
Reinhard Krämer, Sascha Nicklisch, and Vera Ott
4 Studying Mechanosensitive Ion Channels Using Liposomes ...... 31
Boris Martinac, Paul R. Rohde, Andrew R. Battle, Evgeny
Petrov, Prithwish Pal, Alexander Fook Weng Foo, Valeria
Vásquez, Thuan Huynh, and Anna Kloda
5 Studying Amino Acid Transport Using Liposomes ............... 55
Cesare Indiveri
6 Use of Liposomes for Studying Interactions of Soluble
Proteins with Cellular Membranes ............................ 69
Chris T. Höfer, Andreas Herrmann, and Peter Müller
7 Liposomal Reconstitution of Monotopic Integral Membrane
Proteins .................................................... 83
Zahra MirAfzali and David L. De Witt
8 The Reconstitution of Actin Polymerization on Liposomes ..... 95
Mark Stamnes and Weidong Xu
9 Electroformation of Giant Unilamellar Vesicles from Native
Membranes and Organic Lipid Mixtures for the Study of
Lipid Domains under Physiological Ionic-Strength
Conditions ................................................. 105
L.-Ruth Montes, Hasna Ahyayauch, Maitane Ibarguren, Jesus
Sot, Alicia Alonso, Luis A. Bagatolli, and Felix M. Goñi
10 Visualization of Lipid Domain-Specific Protein Sorting
in Giant Unilamellar Vesicles .............................. 115
Martin Stöckl, Jörg Nikolaus, and Andreas Herrmann
11 Biosynthesis of Proteins Inside Liposomes .................. 127
Pasquale Stano, Yutetsu Kuruma, Tereza Pereira de Souza,
and Pier Luigi Luisi
12 Study of Respiratory Cytochromes in Liposomes .............. 147
Iseli L. Nantes, Cintia Kawai, Felipe S. Pessoto, and
Katia C.U. Mugnol
13 Use of Liposomes to Evaluate the Role of Membrane
Interactions on Antioxidant Activity ....................... 167
Salette Reis, Marlene Lúcio, Marcela Segundo, and José
L.F.C. Lima
14 Studying Colloidal Aggregation Using Liposomes ............. 189
Juan Sabín, Gerardo Prieto, and Félix Sarmiento
15 Assessment of Liposome-Cell Interactions ................... 199
Jan A.A.M. Kamps
16 Methods to Monitor Liposome Fusion, Permeability, and
Interaction with Cells ..................................... 209
Nejat Düzgüneş, Henrique Faneca, and Maria С. Pedroso de
Lima
17 The Use of Isothermal Titration Calorimetry to Study
Multidrug Transport Proteins in Liposomes .................. 233
David Miller and Paula J. Booth
18 Studying Lipid Organization in Biological Membranes Using
Liposomes and EPR Spin Labeling ............................ 247
Witold K. Subczynski, Marija Raguz, and Justyna Widomska
19 Membrane Translocation Assayed by Fluorescence
Spectroscopy ............................................... 271
J. Broecker and S. Keller
20 Interaction of Lipids and Ligands with Nicotinic
Acetylcholine Receptor Vesicles Assessed by Electron
Paramagnetic Resonance Spectroscopy ........................ 291
Hugo Rubén Arias
21 Environmental Scanning Electron Microscope Imaging of
Vesicle Systems ............................................ 319
Yvonne Perrie, Habib Ali, Daniel J. Kirby, Afzal
U.R. Mohammed, Sarah E. McNeil, and Anil Vangala
22 Freeze-Fracture Electron Microscopy on Domains in Lipid
Mono- and Bilayer on Nano-Resolution Scale ................. 333
Brigitte Papahadjopoulos-Sternberg
23 Atomic Force Microscopy for the Characterization of
Proteoliposomes ............................................ 351
Johannes Sitterberg, Maria Manuela Gaspar, Carsten
Ehrhardt, and Udo Bakowsky
24 Method of Simultaneous Analysis of Liposome Components
Using HPTLC/FID ............................................ 363
Sophia Hatziantoniou and Costas Demetzos
25 Viscometric Analysis of DNA-Lipid Complexes ................ 369
Sadao Hirota and Nejat Düzgüneş
26 Fluorometric Analysis of Individual Cationic Lipid-DNA
Complexes .................................................. 385
Edwin Pozharski
27 Fluorescence Resonance Energy Transfer-Based Analysis of
Lipoplexes ................................................. 393
Edwin Pozharski
28 Analysis of Lipoplex Structure and Lipid Phase Changes ..... 399
Rumiana Koynova
29 Fluorescence Methods for Evaluating Lipoplex-Mediated
Gene Delivery .............................................. 425
Henrique Faneca, Nejat Düzgüneş, and Maria C. Pedroso de
Lima
30 FRET Imaging of Cells Transfected with siRNA/Liposome
Complexes .................................................. 439
Il-Han Kim, Anne Järve, Markus Hirsch, RoЯer Fischer,
Michael F. Trendelenburg, Ulrich Massing, Karl Rohr, and
Mark Helm
31 Spectral Bio-Imaging and Confocal Imaging of the
Intracellular Distribution of Lipoplexes ................... 457
Sebastian Schneider and Regine Süss
32 Techniques for Loading Technetium-99m and Rhenium-186/188
Radionuclides into Pre-formed Liposomes for Diagnostic
Imaging and Radionuclide Therapy ........................... 469
Beth Goins, Ande Bao, and William T. Phillips
33 Fluorescence Correlation Spectroscopy for the Study of
Membrane Dynamics and Organization in Giant Unilamellar
Vesicles ................................................... 493
Ana J. Garcia-Sáez, Dolores C. Carrer, and Petra Schwille
34 Liposome Biodistribution via Europium Complexes ............ 509
Nathalie Mignet and Daniel Scherman
35 Biosensor-Based Evaluation of Liposomal Binding Behavior ... 519
Gerd Bendas
36 Use of Liposomes to Study Vesicular Transport .............. 531
Kohji Takei, Hiroshi Yamada, and Tadashi Abe
Index ......................................................... 543
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