Mouse cell culture: methods and protocols (New York, 2010). - ОГЛАВЛЕНИЕ / CONTENTS
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ОбложкаMouse cell culture: methods and protocols / ed. by A.Ward, D.Tosh. - New York: Humana Press, 2010. - xi, 257 p.: ill. (some col.). - (Methods in molecular biology; 633) (Springer protocols). - ISBN 978-1-58829-772-3; ISSN 1064-3745
 

Оглавление / Contents
 
Preface ......................................................... v
Contributors ................................................... ix
1  The Culture of Mouse Embryonic Stem Cells and Formation
   of Embryoid Bodies ........................................... 1
   Melany Jackson, A. Helen Taylor, Elizabeth A. Jones, and
   Lesley M. Forrester
2  Derivation of Primary Mouse Embryonic Fibroblast (PMEF)
   Cultures .................................................... 19
   Alastair S. Garfield
3  Embryonic Skeletal Muscle Microexplant Culture and
   Isolation of Skeletal Muscle Stem Cells ..................... 29
   Janet Smith and Deborah Merrick
4  The Embryonic Kidney: Isolation, Organ Culture,
   Immunostaining and RNA Interference ......................... 57
   Jamie A. Davies
5  Explant Culture of Mouse Embryonic Whole Lung, Isolated
   Epithelium, or Mesenchyme Under Chemically Defined
   Conditions as a System to Evaluate the Molecular
   Mechanism of Branching Morphogenesis and Cellular 
   Differentiation ............................................. 71
   Pierre-Marie del Moral and David Warburton
6  Isolation, Culture, and Characterisation of Mouse
   Embryonic Oesophagus and Intestine .......................... 81
   Jonathan M. Quinlan, Wei-Yuan Yu, and David Tosh
7  Isolation and Culture of Embryonic Pancreas and Liver ....... 91
   Zoë D. Burke, Wan-Chun Li, Jonathan M.W. Slack, and David
   Tosh
8  Isolation and Culture of Mouse Satellite Cells ............. 101
   Antonio Musarò and Laura Barberi
9  Analysis of Cardiac Myocyte Biology in Transgenic Mice:
   A Protocol for Preparation of Neonatal Mouse Cardiac
   Myocyte Cultures ........................................... 113
   Nigel J. Brand, Enrique Lara-Pezzi, Nadia Rosenthal, and
   Paul J.R. Barton
10 Short- and Long-Term Cultivation of Embryonic and 
   Neonatal Murine Keratinocytes .............................. 125
   Reto Caldelari and Eliane J. Müller
11 Isolation, Culture and Analysis of Mouse Mammary
   Epithelial Cells ........................................... 139
   Matthew J. Smalley
12 Isolation and Culture of Mouse Pancreatic Islets for Ex
   Vivo Imaging Studies with Trappable or Recombinant
   Fluorescent Probes ......................................... 171
   Magalie A. Ravier and Guy A. Rutter
13 Isolation and Culture of Adult Mouse Hepatocytes ........... 185
   Wan-Chun Li, Kate L. Ralphs, and David Tosh
14 Isolation and Culture of Mouse Intestinal Cells ............ 197
   Charles Frederick Campbell
15 Derivation of Primary Choroid Plexus Epithelial Cells
   from the Mouse ............................................. 207
   Trevelyan R. Menheniott, Marika Charalambous, and Andrew
   Ward
16 The Preparation of Primary Cortical Neuron Cultures and
   a Practical Application Using Immunofluorescent
   Cytochemistry .............................................. 221
   Carla Sciarretta and Liliana Minichiello
17 Cell Culture of Primary Cerebellar Granule Cells ........... 233
   Dana Krämer and Liliana Minichiello
18 Isolation and Generation of Neurosphere Cultures from
   Embryonic and Adult Mouse Brain ............................ 241
   Henrik Ahlenius and Zaal Kokaia

Subject Index ................................................. 253


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