Preface ......................................................... v
Contributors ................................................... ix
1 Molecular Methods to Study Complex Microbial Communities ..... 1
José M. Vieites, María-Еugeniа Guazzaroni, Ana Beloqui,
Peter N. Golyshin, and Manuel Ferrer
2 Construction of Small-Insert and Large-Insert Metagenomic
Libraries ................................................... 39
Carola Simon and Rolf Daniel
3 Construction and Screening of Marine Metagenomic Libraries .. 51
Nancy Wetland, Carolin Löscher, Rebekka Metzger, and Ruth
Schmitz
4 Metagenomic Analysis of Isotopically Enriched DNA ........... 67
Yin Chen, Josh D. Neufeld, Marc G. Dumont, Michael
W. Friedrich, and J. Colin Murrell
5 Wide Host-Range Cloning for Functional Metagenomics ......... 77
Margaret Wexler and Andrew W.B. Johnston
6 Cloning and Expression Vectors for a
Gram-Positive Host, Streptomyces lividans ................... 97
Kristof Vrancken, Lieve Van Mellaert, and Jozef Anné
7 Heterologous Gene Expression in the Hyperthermophilic
Archaeon Sulfolobus solfataricus ........................... 109
Angel Angelov and Wolfgang Liebl
8 Novel Tools for the Functional Expression of Metagenomic
DNA ........................................................ 117
Sonja Christina Troeschel, Thomas Drepper, Christian
Leggewie, Wolfgang R. Streit, and Karl-Erich Jaeger
9 Screening of Functional Promoter from Metagenomic
DNA for Practical Use in Expression Systems ................ 141
So-Youn Park and Geun-Joong Kim
10 Substrate-Induced Gene Expression Screening: A Method
for High-Throughput Screening of Metagenome Libraries ...... 153
Taku Uchiyama and Kentaro Miyazaki
11 Screens for Active and Stereoselective Hydrolytic Enzymes .. 169
Dominique Böttcher, Marten Schmidt, and Uwe
T. Bornscheuer
12 Screening for Cellulase-Encoding Clones in Metagenomic
Libraries .................................................. 177
Nele Ilmberger and Wolfgang R. Streit
13 Screening Metagenomic Libraries for Laccase Activities ..... 189
Manuel Ferrer, Ana Beloqui, and Peter N. Golyshin
14 Screening for N-AHSL-Based-Signaling Interfering Enzymes ... 203
Phil M. Oger and Stéphane Uroz
15 Identification of Molecular Markers to Follow Up the
Bioremediation of Sites Contaminated with Chlorinated
Compounds .................................................. 219
Massimo Marzorati, Annalisa Balloi, Francesca De Ferra,
and Daniele Daffonchio
16 Methods for the Isolation of Genes Encoding Novel PHB
Cycle Enzymes from Complex Microbial Communities ........... 235
Ricardo F. Nordeste, Maria A. Trainer, and Trevor
C. Charles
17 Metagenomic Approaches to Identify and Isolate Bioactive
Natural Products from Microbiota of Marine Sponges ......... 247
Cristian Gurgui and Jörn Piel
18 Screening for Novel Antibiotic Resistance Genes ............ 265
Mindy G. Brown, Elizabeth H. Mitchell, and David
L. Balkwill
19 Novel Metal Resistance Genes from Microorganisms:
A Functional Metagenomic Approach .......................... 273
José E. González-Pastor and Salvador Mirete
20 Retrieval of Full-Length Functional Genes Using
Subtractive Hybridization Magnetic Bead Capture ............ 287
Tracy Meiring, Inonge Mulako, Maria I. Tujfin, Quintan
Meyer, and Donald A. Cowan
21 Detection and Isolation of Selected Genes of Interest
from Metagenomic Libraries by a DNA Microarray Approach .... 299
Gopal P. Pathak and Wolfgang Gärtner
22 Application of DNA Microarray for Screening Metagenome
Library Clones ............................................. 313
Soo-Je Park, Jong-Chan Chae, and Sung-Keun Rhee
23 MetaGenomeThreader: A Software Tool for Predicting
Genes in DNA-Sequences of Metagenome Projects .............. 325
David J. Schmitz-Hübsch and Stefan Kurtz
Index ......................................................... 339
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