Preface ......................................................... v
Contributors ................................................... xi
1 A Novel Bacterial Expression Method with Optimized
Parameters for Very High Yield Production of Triple-
Labeled Proteins ............................................. 1
Victoria Murray, Yuefei Huang, Jianglei Chen, Jianjun
Wang, and Qianqian Li
2 Isotopic Labeling of Heterologous Proteins in the Yeast
Pichia pastoris and Kluyveromyces lactis .................... 19
Toshihiko Sugiki, Osamu Ichikawa, Mayumi Miyazawa-Onami,
Ichio Shimada, and Hideo Takahashi
3 Isotope Labeling in Insect Cells ............................ 37
Krishna Saxena, Arpana Dutta, Judith Klein-Seetharaman,
and Harald Schwalbe
4 Isotope Labeling in Mammalian Cells ......................... 55
Arpana Dutta, Krishna Saxena, Harald Schwalbe, and Judith
Klein-Seetharaman
5 Cell-Free Protein Production for NMR Studies ................ 71
Mitsuhiro Takeda and Masatsune Kainosho
6 Cell-Free Membrane Protein Expression for Solid-State NMR ... 85
Alaa Abdine, Kyu-Ho Park, and Dror E. Warschawski
7 Expression and Purification of Src-family Kinases for
Solution NMR Studies ....................................... 111
Andrea Piserchio, David Cowburn, and Ranajeet Ghose
8 NMR Studies of Large Protein Systems ....................... 133
Shiou-Ru Tzeng, Ming-Tao Pai, and Charalampos
G. Kalodimos
9 Protein Dynamics by 15N Nuclear Magnetic Relaxation ........ 141
Fabien Ferrage
10 Bacterial Production and Solution NMR Studies of a Viral
Membrane Ion Channel ....................................... 165
Jolyon K. Claridge and Jason R. Schnell
11 Preparation of the Modular Multi-Domain Protein RPA for
Study by NMR Spectroscopy .................................. 181
Chris A. Brosey, Marie-Eve Chagot, and Walter J. Chazin
12 NMR Studies of Protein-RNA Interactions .................... 197
Carta A. Theimer, Nakesha L. Smith, and May Khanna
13 Preparation and Optimization of Protein-DNA Complexes
Suitable for Detailed NMR Studies .......................... 219
My D. Sam and Robert T. Clubb
14 NMR Studies of Protein-Ligand Interactions ................. 233
Michael Goldflam, Teresa Tarragó, Margarida Gairí, and
Ernest Giralt
15 In-Cell NMR Spectroscopy in Escherichia coli ............... 261
Kirsten E. Robinson, Patrick N. Reardon, and Leonard
D. Spicer
16 Deuterated Peptides and Proteins: Structure and Dynamics
Studies by MAS Solid-State NMR ............................. 279
Bernd Reif
17 Solid-State NMR Spectroscopy of Protein Complexes .......... 303
Shangjin Sun, Yun Han, Sivakumar Paramasivam, Si Yan,
Amanda E. Siglin, John C. Williams, In-Ja L. Byeon, Jinwoo
Ahn, Angela M. Gronenborn, and Tatyana Polenova
18 Synthesis, Purification, and Characterization of Single
Helix Membrane Peptides and Proteins for NMR Spectroscopy .. 333
Miki Itaya, Ian C. Brett, and Steven O. Smith
19 Assignment of Backbone Resonances in a Eukaryotic Protein
Kinase - ERK2 as a Representative Example .................. 359
Andrea Piserchio, Kevin N. Dalby, and Ranajeet Ghose
20 Electrostatics of Hydrogen Exchange for Analyzing Protein
Flexibility ................................................ 369
Gyiselda Hernández, Janet S. Anderson, and David
M. LeMaster
21 Fast Protein Backbone NMR Resonance Assignment Using
the BATCH Strategy ......................................... 407
Bernhard Brutscher and Ewen Lescop
22 Comprehensive Automation for NMR Structure Determination
of Proteins ................................................ 429
Paul Guerry and Torsten Herrmann
23 ARIA for Solution and Solid-State NMR ...................... 453
Benjamin Bardiaux, Thérèse Malliavin, and Michael Nilges
24 Determining Protein Dynamics from 15N Relaxation Data
by Using DYNAMICS .......................................... 485
David Fushman
Index ......................................................... 513
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