Preface ......................................................... v
Contributors ................................................... ix
1 Introduction: Strategies for Developing Genetically
Modified Mice ................................................ 1
Marten H. Hofker
2 Genetic Modification of the Mouse: General Technology -
Pronuclear and Blastocyst Injection ......................... 11
Jan Willem Voncken
3 Generation of Chimeras by Aggregation of Embryonic Stem
Cells with Diploid or Tetraploid Mouse Embryos .............. 37
Jérôme Artus and Anna-Katerina Hadjantonakis
4 Cryopreservation of Mouse Spermatozoa and In Vitro
Fertilization ............................................... 57
Naomi Nakagata
5 Autopsy and Histological Analysis of the Transgenic Mouse ... 75
Marion J.J. Gijbels and Menno P.J. de Winther
6 Transgene Design ............................................ 89
Bart van de Sluis and Jan Willem Voncken
7 Inducible Transgenic Mouse Models .......................... 103
Thomas L. Saunders
8 Lentiviral Transgenesis .................................... 117
Terunaga Nakagawa and Casper C. Hoogenraad
9 Transgenesis in Mouse Embryonic Stem Cells ................. 143
Janine van Ree, Wei Zhou, Ming Li, and Jan M. van Deursen
10 Engineering ВАС Reporter Gene Constructs for Mouse
Transgenesis ............................................... 163
Yu Fu and Peter Maye
11 Targeting Vector Construction Through Recombineering ....... 181
Liviu A. Malureanu
12 Generating Conditional Knockout Mice ....................... 205
Roland H. Friedel, Wolfgang Wurst, Benedikt Wefers, and
Ralf Kühn
13 Hypomorphic Mice ........................................... 233
Darren J. Baker
14 MICER Targeting Vectors for Manipulating the Mouse Genome .. 245
Chunhong Liu, Paul F. Szurek, and Y. Eugene Yu
15 Knock-In Approaches ........................................ 257
Anton J.M. Roebroek, Philip L.S.M. Gordts, and Sara
Reekmans
16 Generation of a Series of Knock-In Alleles Using RMCE in
ES Cells ................................................... 277
Anton J.M. Roebroek, Philip L.S.M. Gordts, and Sara
Reekmans
17 Selection of Targeted Mutants from a Library of Randomly
Mutagenized ES Cells ....................................... 283
Kyoji Horie, George Gaitanaris, and Alexander Gragerov
18 Generation of Genetically Modified Rodents Using Random
ENU Mutagenesis ............................................ 295
Ruben van Boxtel and Edwin Cuppen
19 Bone Marrow Transplantations to Study Gene Function
in Hematopoietic Cells ..................................... 309
Menno P.J. de Winther and Peter Heeringa
20 Adenovirus-Mediated Gene Transfer .......................... 321
Ко Willems van Dijk, Kyriakos E. Kypreos, Frits J.
Fallaux, and Jurre Hageman
Index ......................................................... 345
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