Electron crystallography of soluble and membrane proteins: methods and protocols / ed. by I.Schmidt-Krey, Y.Cheng. - New York: Humana Press, 2013. - xiii, 586 p.: ill. - (Methods in molecular biology; 955) (Springer protocols). - Incl. bibl. ref. - Ind.: p.569-586. - ISBN 978-1-62703-175-2  Место хранения:   056 | Институт химической биологии и фундаментальной медицины CO РАН | Новосибирск| Библиотека

Оглавление / Contents

Preface ......................................................... v Contributors ................................................... xi 1 Introduction to Electron Crystallography ..................... 1 Werner Kühlbrandt 2 Practical Aspects in Expression and Purification of Membrane Proteins for Structural Analysis ................... 17 Kutti R. Vinothkumar, Patricia С. Edwards, and Joerg Standfuss 3 Two-Dimensional Crystallization of Membrane Proteins by Reconstitution Through Dialysis ............................. 31 Matthew C. Johnson, Tina M. Dreaden, Laura Y. Kim, Frederik Rudolph, Bridgette A. Barry, and Ingeborg Schmidt-Krey 4 Monolayer Two-Dimensional Crystallization of Membrane Proteins .................................................... 59 Luc Lebeau and Catherine Vénien-Bryan 5 Screening for Two-Dimensional Crystals by Transmission Electron Microscopy of Negatively Stained Samples ........... 73 Tina M. Dreaden, Maureen Metcalfe, Laura Y. Kim, Matthew C. Johnson, Bridgette A. Barry, and Ingeborg Schmidt-Krey 6 Low Dose Techniques and Cryo-Electron Microscopy ........... 103 Toshinori Fujiyoshi 7 Grid Preparation for Cryo-Electron Microscopy .............. 119 Nobuhiko Gyobu 8 Recording High-Resolution Images of Two-Dimensional Crystals of Membrane Proteins .............................. 129 Agustin Avila-Sakar, Xueming Li, Shawn Q. Zheng, and Yifan Cheng 9 The Collection of High-Resolution Electron Diffraction Data ....................................................... 153 Tamir Gonen 10 Image Processing of 2D Crystal Images ...................... 171 Marcel Arbeit, Daniel Castaño-Díez, Raphael Thierry, Bryant R. Gipson, Xiangyan Zeng, and Henning Stahlberg 11 Merging of Image Data in Electron Crystallography .......... 195 Marcel Arheit, Daniel Castaño-Díez, Raphaël Thierry, Priyanka Abeyrathne, Bryant R. Gipson, and Henning Stahlberg 12 Evaluation of Electron Crystallographic Data from Images of Two-Dimensional Crystals ................................ 211 Vinzenz M. Unger 13 Modeling, Docking, and Fitting of Atomic Structures to 3D Maps from Cryo-Electron Microscopy ...................... 229 Gregory S. Allen and David L. Stokes 14 Phasing Electron Diffraction Data by Molecular Replacement: Strategy for Structure Determination and Refinement ................................................. 243 Goragot Wisedchaisri and Tamir Gonen 15 High-Throughput Methods for Electron Crystallography ....... 273 David L. Stokes, Iban Ubarretxena-Belandia, Tamir Gonen, and Andreas Engel 16 Automated Grid Handling and Image Acquisition for Two- Dimensional Crystal Screening .............................. 297 Anchi Cheng 17 Automation of Data Acquisition in Electron Crystallography ............................................ 307 Anchi Cheng 18 Automation of Image Processing in Electron Crystallography ............................................ 313 Marcel Arheit, Daniel Castaño-Diez, Raphael Thierry, Bryant R. Gipson, Xiangyan Zeng, and Henning Stahlberg 19 Choice and Maintenance of Equipment for Electron Crystallography ............................................ 331 Deryck J. Mills and Janet Vonck 20 Future Developments in Instrumentation for Electron Crystallography ............................................ 353 Kenneth H. Downing 21 Tubular Crystals and Helical Arrays: Structural Determination of HIV-1 Capsid Assemblies Using Iterative Helical Real-Space Reconstruction .......................... 381 Peijun Zhang, Xin Meng, and Gongpu Zhao 22 Single Particle Electron Microscopy ........................ 401 Wilson C.Y. Lau and John L. Rubinstein 23 Electron Tomography of Paracrystalline 2D Arrays ........... 427 Hanspeter Winkler, Shenping Wu, and Kenneth A. Taylor 24 High-Resolution Imaging of 2D Outer Membrane Protein F. Crystals by Atomic Force Microscopy ..................... 461 Dimitrios Fotiadis and Daniel J. Müller 25 Determination of Soluble and Membrane Protein Structures by X-Ray Crystallography ................................... 475 Raquel L. Lieberman, Mary E. Peek, and J. Derrick Watkins 26 Solution Nuclear Magnetic Resonance Spectroscopy ........... 495 James J.С. ho и and Remy Sounier 27 Structure-Function Insights of Membrane and Soluble Proteins Revealed by Electron Crystallography .............. 519 Tina M. Dreaden, Bharanidharan Devarajan, Bridgette A. Barry, and Ingeborg Schmidt-Krey 28 Lipid Monolayer and Sparse Matrix Screening for Growing Two-Dimensional Crystals for Electron Crystallography: Methods and Examples ....................................... 527 Mark Yeager, Kelly A. Dryden, and Barbie K. Ganser- Pornillos 29 Processing of Electron Diffraction Patterns with the XDP Program .................................................... 539 Kaoru Mitsuoka 30 Future Directions of Electron Crystallography .............. 551 Yoshinori Fujiyoshi Index ......................................................... 569