Preface ......................................................... v
Contributors ................................................... ix
1 Basic Immunocytochemistry for Light Microscopy ............... 1
Susan A. Brooks
2 Lectin Histochemistry to Detect Altered Glycosylation in
Cells and Tissues ........................................... 31
Susan A. Brooks and Debbie M.S. Hall
3 Histopathological Assessment of Metastasis .................. 51
Derek E. Roskell and Ian D. Buley
4 Immunocytochemical Detection and Characterisation of
Individual Micrometastatic Tumour Cells ..................... 63
Stephan Braun and Klaus Pantel
5 The Polymerase Chain Reaction ............................... 71
Hazel M. Welch
6 Sodium Dodecyl Sulphate-Polyacrylamide Denaturing Gel
Electrophoresis and Western Blotting Techniques ............. 89
Christine Blancher and Rob Mc Cormick
7 2-DE-Based Proteomics for the Analysis of Metastasis-
Associated Proteins ........................................ 111
Miriam Dwek and Diluka Peiris
8 Assessment of Gelatinases (MMP-2 and MMP-9) by Gelatin
Zymography ................................................. 121
Marta Toth, Anjum Sohail, and Rafael Fridman
9 Determination of Cell-Specific Receptor Binding using
a Combination of Immunohistochemistry and in vitro
Autoradiography: Relevance to Therapeutic Receptor
Targeting in Cancer ........................................ 137
Michael R. Dashwood and Marilena Loizidou
10 Fluorescence In Situ Hybridization for Cancer-Related
Studies .................................................... 149
Lyndal Kearney and Janet Shipley
11 Using a Quartz Crystal Microbalance Biosensor for the
Study of Metastasis Markers on Intact Cells ................ 175
Julien Saint-Guirons and Björn Ingemarsson
12 Cell Separations by Flow Cytometry ......................... 185
Derek Davies
13 Detection of Putative Cancer Stem Cells of the Side
Population Phenotype in Human Tumor Cell Cultures .......... 201
Matthias Christyen, Matthias Ballmaier, Ulrich Lehmann,
and Hans Kreipe
14 Genes Involved in the Metastatic Cascade of Medullary
Thyroid Tumours ............................................ 217
Caroline Schreiber, Kirsten Vormbrock and Ulrike Ziebold
15 High-resolution Quantitative Methylation Analysis of
microRNA Genes using PyrosequencingTM ...................... 229
Ulrich Lehmann, Cord Albat, and Hans Kreipe
16 RNAi Technology to Block the Expression of Molecules
Relevant to Metastasis: The Cell Adhesion Molecule
CEACAM1 as an Instructive Example .......................... 241
Daniel Wicklein
17 GaIectin-3 Binding and Metastasis .......................... 251
Pratima Nangia-Makker, Vitaly Balan, and Avraham Raz
18 Lectin Array-Based Strategies for Identifying Metastasis-
Associated Changes in Glycosylation ........................ 267
Simon Fry, Babak Afrough, Anthony Leathern, and Miriam
Dwek
Index ......................................................... 273
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