Preface ......................................................... v
Contributors ................................................... ix
1 Toxicological Aspects for Nanomaterial in Humans ............. 1
Maria Dusinska, Zuzana Magdolenova, and Lise Marie Fjellsbø
2 Lactate Dehydrogenase Assay for Assessment of Polycation
Cytotoxicity ................................................ 13
Ladan Parhamifar, Helene Andersen, and S. Moein Moghimi
3 Combined Fluorimetric Caspase 3/7 Assay and Bradford
Protein Determination for Assessment of Polycation-
Mediated Cytotoxicity ....................................... 23
Anna K. Larsen, Arnaldur Hall, Henrik Lundsgart, and
S. Moein Moghimi
4 Anti-PEG IgM Production via a PEGylated Nano-Carrier
System for Nucleic Acid Delivery ............................ 35
Masako Ichihara, Naoto Moriyoshi, Amr S. Abu Lila,
Tatsuhiro Ishida, and Hiroshi Kiwada
5 Near-Infrared Optical Imaging of Nucleic Acid Nanocarriers
In Vivo ..................................................... 49
Claire Rome, Julien Gravier, Marie Morille, Gilles Divita,
Anne-Laure Bolcato-Bellemin, Véronique Josserand, and
Jean-Luc Coll
6 Lipids for Nucleic Acid Delivery: Synthesis and Particle
Formation ................................................... 67
Michel Bessodes and Nathalie Mignet
7 Histidine-Rich Cationic Amphipathic Peptides for Plasmid
DNA and siRNA Delivery ...................................... 85
Antoine Kichler, A. James Mason, Arnaud Marquette, and
Burkhard Bechinger
8 Synthesis of Polyethylenimine-Based Nanocarriers for
Systemic Tumor Targeting of Nucleic Acids .................. 105
Wolfgang Rödl, David Schaffert, Ernst Wagner, and Manfred
Ogris
9 Synthesis of Bioreducible Polycations with Controlled
Topologies ................................................. 121
Ye-Zi You,Jun-Jie Yan, Zhi-Qiang Yu, and David Oupicky
10 Lyophilization of Synthetic Gene Carriers .................. 133
Julia Christina Kasper, Sarah Küchler, and Wolfgang
Friess
11 Surface-and Hydrogel-Mediated Delivery of Nucleic Acid
Nanoparticles .............................................. 149
Angela K. Pannier and Tatiana Segura
12 Layer-by-Layer Assembled Gold Nanoparticles for the
Delivery of Nucleic Acids .................................. 171
Eva-Christina Wurster, Asmaa Elbakry, Achim Göpferich,
and Miriam Breunig
13 In Situ AFM Analysis Investigating Disassembly of DNA
Nanoparticles and Nano-Films ............................... 183
Yi Zou, Lei Wan, Jenifer Blacklock, David Oupicky, and
Guangzhao Mao
14 Enhancing Nucleic Acid Delivery with Ultrasound and
Microbubbles ............................................... 195
Steven K. Cool, Bart Geers, Ine Lentacker, Stefaan C.
De Smedt, and Niek N. Sanders
15 Magnetic and Acoustically Active Microbubbles Loaded with
Nucleic Acids for Gene Delivery ............................ 205
Dialechti Vlaskou, Christian Plank, and Olga Mykhaylyk
16 Synthesis of Lipidic Magnetic Nanoparticles for Nucleic
Acid Delivery .............................................. 243
Yoshihisa Namiki
17 Lipopeptide Delivery of siRNA to the Central Nervous
System ..................................................... 251
Mark D. Zabel
18 Flow Cytometry-Based Cell Type-Specific Assessment of
Target Regulation by Pulmonary siRNA Delivery .............. 263
Olivia M. Merkel, Leigh M. Marsh, Holger Garn, and Thomas
Kissel
19 Liver-Targeted Gene Delivery Through Retrograde
Intrabiliary Infusion ...................................... 275
Xuan Jiang, Yong Ren, John-Michael Williford, Zhiping Li,
and Hai-Quan Mao
Index ......................................................... 285
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