Nitrogen fixation: methods and protocols / ed. by M.W.Ribbe. - New York: Humana Press, 2011. - x, 326 p.: ill. - (Methods in molecular biology; 766) (Springer protocols). - Incl. bibl. ref. - Ind.: p.313-326. - ISBN 978-1-61779-193-2; ISSN 1064-3745  Место хранения:   03 | Центральная библиотека Иркутского научного центра СО РАН | Иркутск

Оглавление / Contents

Preface ......................................................... v Contributors ................................................... ix Section I Nitrogen Fixation 1 Historic Overview of Nitrogenase Research .................... 3 Yilin Нu and Mark us W. Ribbe 2 Mechanism of Mo-Dependent Nitrogenase ........................ 9 Zhi-Yong Yang, Karamatullah Danyal, and Lance C. Seefeldt 3 Assembly of Nitrogenase MoFe Protein ........................ 31 Chi-Chung Lee, Aaron W. Fay, Jared A. Wiig, Markus W. Ribbe, and Yilin Нu 4 Genomic Analysis of Nitrogen Fixation ....................... 49 Ina P. O'Carroll and Patricia C. Dos Santos 5 Enzymatic Systems with Homology to Nitrogenase .............. 67 Jürgen Moser and Markus J. Bröcker Section II Genetic and Biochemical Methods 6 Molecular Biology and Genetic Engineering in Nitrogen Fixation .................................................... 81 Patricia C. Dos Santos 7 Purification of Nitrogenase Proteins ........................ 93 Jared A. Wiig, Chi-Chung Lee, Aaron W. Fay, Yilin Нu, and Markus W. Ribbe 8 Assays of Nitrogenase Reaction Products .................... 105 William E. Newton and Michael J. Dilworth 9 Methods for Nitrogenase-Like Dark Operative Protochlorophyllide Oxidoreductase ......................... 129 Jürgen Moser and Markus J. Bröcker Section III Spectroscopic Methods 10 X-Ray Crystallography ...................................... 147 Lauren E. Roth and F. Akif Tezcan 11 X-Ray Absorption Spectroscopy .............................. 165 Serena DeBeer 12 Small Angle X-Ray Scattering Spectroscopy .................. 177 David W. Mulder and John W. Peters 13 Electron Paramagnetic Resonance Spectroscopy ............... 191 Karamatullah Danyal, Zhi-Yong Yang, and Lance C. Seefeldt 14 Magnetic Circular Dichroism Spectroscopy ................... 207 Brian J. Hales 15 Mossbauer Spectroscopy ..................................... 221 Boi Hanh Huynh Section IV Chemical Methods 16 Protocols for Cofactor Isolation of Nitrogenase ............ 239 Aaron W. Fay, Cbi-Cbung Lee, Jared A. Wiig, Yilin Нu, and Markus W. Ribbe 17 Techniques for Functional and Structural Modeling of Nitrogenase ................................................ 249 Patrick L. Holland Section V Theoretical Calculations 18 Nitrogenase Structure and Function Relationships by Density Functional Theory .................................. 267 Travis V. Harris and Robert K. Szilagyi 19 Modeling the MoFe Nitrogenase System with Broken Symmetry Density Functional Theory .................................. 293 Gregory M. Sandala and Louis Noodleman Index ......................................................... 313