Preface ......................................................... v
Contributors ................................................... ix
1 Real-Time Quantitative PCR, Pathogen Detection and MIQE ...... 1
Gemma Johnson, Tania Nolan, and Stephen A. Bustin
2 Overcoming Inhibition in Real-Time Diagnostic PCR ........... 17
Johannes Hedman and Peter Bådström
3 Quality in the Molecular Microbiology Laboratory ............ 49
Paul S. Wallace and William G. MacKay
4 Pre-analytical Sample Treatment and DNA Extraction
Protocols for the Detection of Bacterial Pathogens from
Whole Blood ................................................. 81
Wendy L.J. Hansen, Cathrien A. Bruggeman, and Petra
F.G. Wolffs
5 Detection of Bacterial Contamination in Platelet
Concentrates Using Flow Cytometry and Real Time PCR
Methods ..................................................... 91
Tanja Vollmer, Knut Kleesiek, and Jens Dreier
6 Multiplex Real-Time PCR Assay for the Detection of
Meticillin-Resistant Staphylococcus aureus and Panton-
Valentine Leukocidin from Clinical Samples ................. 105
Lynne Renwick, Anne Holmes, and Kate Templeton
7 PCR Detection of Haemophilus influenzae from Respiratory
Specimens .................................................. 115
Guma M.K. Abdeldaim and Bjorn Herrmann
8 Rapid Detection of Atypical Respiratory Bacterial
Pathogens by Real-Time PCR ................................. 125
Clare L. Ling and Timothy D. McHugh
9 Utilization of Multiple Real-Time PCR Assays for the
Diagnosis of Bordetella spp. in Clinical Specimens ......... 135
Kathleen M. Tatti and Maria Lucia Tondella
10 Detection of Mycoplasma pneumoniae by Real Time PCR ........ 149
Jonas M. Winchell and Stephanie L. Mitchell
11 Real-Time PCR Assay for the Diagnosis of Pneumocystis
jirovecii Pneumonia ........................................ 159
Judith Fillaux and Antoine Berry
12 Rapid Identification of Mycobacteria and Rapid Detection
of Drug Resistance in Mycobacterium tuberculosis in
Cultured Isolates and in Respiratory Specimens ............. 171
Wing-Cheong Yam and Kit-Hang Gilman Siu
13 Direct Detection of Mycobacterium ulcerans in Clinical
Specimens and Environmental Samples ........................ 201
Caroline J. Lavender and Janet A.M. Fyfe
14 Detection of Bartonella spp. DNA in Clinical Specimens
Using an Internally Controlled Real Time PCRAssay .......... 217
Anneke M.C. Bergmans and John W.A. Rossen
15 Simultaneous Direct Identification of Genital
Microorganisms in Voided Urine Using Multiplex PCR-Based
Reverse Line Blot Assays ................................... 229
Michelle L. McKechnie, Fanrong Kong, and Gwendolyn
L. Gilbert
16 Diagnosis of Clostridium difficile Infection Using Real-
Time PCR ................................................... 247
Renate Johanna van den Berg, Dennis Bakker, and Ed
J. Kuijper
17 Detection of Pathogenic Leptospira spp. Through Real-Time
PCR (qPCR) Targeting the LipL32 Gene ....................... 257
Robyn Anne Stoddard
18 Sensitive and Rapid Detection of Campylobacter jejuni
and Campylobacter coli Using Loop-Mediated Isothermal
Amplification .............................................. 267
Wataru Yamazaki
19 PCR Detection of Helicobacter pylori in Clinical Samples ... 279
Emiko Rimbara, Masanori Sasatsu, and David Y. Graham
20 Rapid Detection of the Escherichia coli Genospecies in
Water by Conventional and Real-Time PCR .................... 289
Andrée F. Maheux, Luc Bissonnette, and Michel G. Bergeron
21 Multiplex Real-Time PCR (MRT-PCR) for Diarrheagenic ........ 307
Francesca Barletta, Theresa J. Ochoa, and Thomas G.
Cleary
Index ......................................................... 315
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