Virus-host interactions: methods and protocols / ed. by S.M.Bailer, D.Lieber. - New York: Springer Science+Business Media: Humana press, 2013. - ix, 372 p.: ill., tab. - (Methods in molecular biology; 1064) (Springer protocols). - Bibliogr. at the end of the chapters. - Ind.: p.367-372. - ISBN 978-1-62703-600-9; ISSN 1064-3745 Шифр: (Pr 852/N 1064) 02  Место хранения:   02 | Отделение ГПНТБ СО РАН | Новосибирск

Оглавление / Contents

Preface ......................................................... v Contributors ................................................... ix 1 A High-Throughput Yeast Two-Hybrid Protocol to Determine Virus-Host Protein Interactions .............................. 1 Hannah Striebinger, Manfred Koegl, and Susanne M. Bailer 2 Analysis of Protein-Protein Interactions Using LUMIER Assays ...................................................... 17 Sonja Blasche and Manfred Koegl 3 Detection of Protein Interactions During Virus Infection by Bimolecular Fluorescence Complementation ................. 29 Stefan Becker and Jens von Einem 4 Discovery of Host-Viral Protein Complexes During Infection .. 43 Daniell L. Rowles, Scott S. Terbune, and Ileana M. Cristea 5 Screening for Host Proteins with Pro- and Antiviral Activity Using High-Throughput RNAi ......................... 71 Samantha J. Griffiths 6 High Resolution Gene Expression Profiling of RNA Synthesis, Processing, and Decay by Metabolic Labeling of Newly Transcribed RNA Using 4-Thiouridine ................... 91 Lars Dölken 7 Isolation and Characterization of Pathogen-Bearing Endosomes Enable Analysis of Endosomal Escape and Identification of New Cellular Cofactors of Infection ...... 101 Konstanze D. Scheffer, Ruth Popa-Wagner, and Luise Florin 8 Computational Analysis of Virus-Host Interactomes .......... 115 Caroline C. Friedel 9 Interspecies Heterokaryon Assay to Characterize the Nucleocytoplasmic Shuttling of Herpesviral Proteins ........ 131 Shuai Wang, Kezheng Wang, and Chunfu Zheng 10 Detection of Integrated Herpesvirus Genomes by Fluorescence In Situ Hybridization (FISH) .................. 141 Benedikt B. Kaufer 11 Fast Generation of Stable Cell Lines Expressing Fluorescent Marker Molecules to Study Pathogen Induced Processes .................................................. 153 Jens Bernhard Bosse, Jessica Rogues, and Harald Wodrich 12 Determination of HSV-1 Infectivity by Plaque Assay and a Luciferase Reporter Cell Line ............................ 171 Diana Lieber and Susanne M. Bailer 13 Generation of a Stable Cell Line for Constitutive miRNA Expression ................................................. 183 Diana Lieber 14 Applications for a Dual Fluorescent Human Cytomegalovirus in the Analysis of Viral Entry ............................. 201 Kerstin Laib Sampaio, Gerhard Jahn, and Christian Sinzger 15 High Temporal Resolution Imaging Reveals Endosomal Membrane Penetration and Escape of Adenoviruses in Real Time ....................................................... 211 Ruben Martinez, Andrew M. Burrage, Christopher M. Wiethoff, and Harald Wodrich 16 Three-Dimensional Visualization of Virus-Infected Cells by Serial Sectioning: An Electron Microscopic Study Using Resin Embedded Cells ....................................... 227 Martin Schauflinger, Clarissa Villinger, and Paul Walther 17 3D-Tissue Model for Herpes Simplex Virus-1 Infections ...... 239 Ina Hogk, Steffen Rupp, and Anke Burger-Kentischer 18 In Vivo Visualization of Encephalitic Lesions in Herpes Simplex Virus Type 1 (HSV-1) Infected Mice by Magnetic Resonance Imaging (MRI) .................................... 253 Wali Hafezi and Verena Hoerr 19 Detection of Antigen-Specific T Cells Based on Intracellular Cytokine Staining Using Flow-Cytometry ....... 267 Tina Schmidt and Martina Sester 20 Functional NK Cell Cytotoxicity Assays Against Virus Infected Cells ............................................. 275 Rebecca J. Aicheler and Richard J. Stanton 21 Assessment of Natural Killer Cell Responses to Human Cytomegalovirus-Infected Macrophages ....................... 289 Zeguang Wu, Giada Frascaroli, and Thomas Mertens 22 Analysis of Cell Migration During Human Cytomegalovirus (HCMV) Infection ........................................... 299 Stefania Varani and Giada Frascaroli 23 Gene Targeting in Mice: A Review ........................... 315 Hicham Bouabe and Klaus Okkenhaug 24 A Protocol for Construction of Gene Targeting Vectors and Generation of Homologous Recombinant Embryonic Stem Cells ...................................................... 337 Hicham Bouabe and Klaus Okkenhaug 25 Measurement of Mouse Cytomegalovirus-Induced Interferon-β with Immortalized Luciferase Reporter Cells ................ 355 Fvgenia Scheibe, Stefan Lienenklaus, Tobias May, Vladimir Gonçalves Magalhães, Siegfried Weiss, and Melanie M. Brinkmann Index ......................................................... 367