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ОбложкаDusny Ch. Microfluidics enable quantitative physiology of individual microorganisms in controlled extracellular environments: Diss. - Dr.-Ing. - Aachen: Shaker, 2015. - xv, 234 p.: ill. - (Chemical biotechnology; vol.21). - Bibliogr.: p.208-228. - ISBN 978-3-8440-3884-2; ISSN 1868-0283
Шифр: (И/Е4-D93) 02
 

Место хранения: 02 | Отделение ГПНТБ СО РАН | Новосибирск

Оглавление / Contents
 
Danksagung ..................................................... IV
Summary ........................................................ IX
Zusammenfassung ................................................. X
List of Abbreviations .......................................... XI
List of Symbols ............................................... XLV
ьst of Cells ................................................... XV

Chapter 1: Introduction ......................................... 1
   General introduction ......................................... 2
   The single cell as the basic biological unit ................. 3
   Single-cell analysis requires environmental control .......... 4
   Physiological consequences of environmental gradients
   and implications for microfluidic single-cell analysis ....... 7
The single-cell continuum ...................................... 10
   Hydrodynamics shape the physical architecture of the
   single-cell boundary environment ............................ 11
   Microfluidic single-cell analysis enables
   environmental control ....................................... 14
Single-cell cultivation in controlled environments with
the Envirostat ................................................. 16
The catalytic black box: How to assess single cells in
a biotechnological context? .................................... 20
Conclusions .................................................... 22
Scope of the thesis ............................................ 23

Chapter 2: Time-resolved single-cell analysis reveals
elevated specific growth rates of isolated microbes and
micropopulations in controlled environments .................... 25
Summary ........................................................ 26
Introduction ................................................... 27
Materials and Methods .......................................... 29
Results and Discussion ......................................... 34
Conclusions .................................................... 40

Chapter 3: Microfluidic control of single yeast
microenvironments discloses ultrasensitive of the mox
promoter to carbon catabolite repression ....................... 41
Summary ........................................................ 42
Introduction ................................................... 43
Materials and Methods .......................................... 46
Results ........................................................ 53
Discussion ..................................................... 68
Conclusions .................................................... 71

Chapter 4: Quantitative measurement of glucose uptake at
a single-cell level ............................................ 73
   Summary ..................................................... 74
   Introduction ................................................ 75
   Materials and Methods ....................................... 79
   Results ..................................................... 85
   Discussion ................................................. 104
   Conclusions ................................................ 108

Chapter 5: The flux probe concept - secreted protein as
a non-disruptive information carrier for I3C-based
metabolic flux analysis ....................................... 111
Summary ....................................................... 112
Introduction .................................................. 113
Materials and Methods ......................................... 116
Results ....................................................... 121
Discussion .................................................... 135
Conclusions ................................................... 138

Chapter 6: Technical bias of microcultivation
environments on single-cell physiology ........................ 139
Summary ....................................................... 140
Introduction .................................................. 141
Materials and Methods ......................................... 146
Results ....................................................... 149
Discussion .................................................... 157
Conclusions ................................................... 160

Chapter 7: General discussion ................................. 161
Introduction .................................................. 162
Beyond the bulk: Analytical concepts for single-cell
analysis ...................................................... 163
Pivotal descriptors for single-cell physiology: linking
cell and environment .......................................... 175
Conclusions ................................................... 188

Chapter 8: Concluding remarks and outlook ..................... 189
Challenging biological limits with microfluidic single-
cell analysis ................................................. 190
Outlook ....................................................... 193

Chapter 9: Appendix ........................................... 195

Chapter 10: References ........................................ 207

Curriculum vitae .............................................. 229

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