Receptor and ion channel detection in the brain: methods and protocols (New York, 2016). - ОГЛАВЛЕНИЕ / CONTENTS
Навигация

Архив выставки новых поступлений | Отечественные поступления | Иностранные поступления | Сиглы
ОбложкаReceptor and ion channel detection in the brain: methods and protocols / ed.: R.Lujan, F.Ciruela. - New York: Humana Press, 2016. - xviii, 475 p.: ill. - (Neuromethods; 110) (Springer protocols). - Bibliogr. at the end of the chapters. - Ind.: p.473-475. - ISBN 978-1-4939-3063-0; ISSN 0893-2336
Шифр: (Pr 1210/110) 02

 

Место хранения: 02 | Отделение ГПНТБ СО РАН | Новосибирск

Оглавление / Contents
 
Series Preface .................................................. v
Preface ....................................................... vii
Contributors ................................................... xv

Part I. Molecular Techniques
1  Production of High-Quality Antibodies for the Study
   of Receptors and Ion Channels ................................ 3
   Masahiko Watanabe
2  Co-immunoprecipitation from Brain ........................... 19
   Xavier Morató, Dasiel O. Borroto-Escuela, Kjell Fuxe,
   Victor Fernández-Dueñas, and Francisco Ciruela
3  Subsynaptic Membrane Fractionation .......................... 31
   Paula M. Canas and Rodrigo A. Cunha
4  Investigation of Neurotransmitter Receptors in Brain 
   Slices Using Cell Surface Biotinylation ..................... 39
   Elek Molnár
5  Single Nanopardcle Tracking of Surface Ion Channels and
   Receptors in Brain Cells .................................... 49
   Juan Varela, Julien Dupuis, and Laurent Groc
6  Radioligand Binding Detection of Receptors in Brain 
   Membranes ................................................... 59
   Fuencisla Pilar-Cuéllar, Alvaro Díaz, Emilio Garro-
   Martínez, Alicia Martín, Beatriz Romero, and Elsa 
   M. Valdizán
7  Recombinant Alphavirus-Mediated Expression of Ion Channels
   and Receptors in the Brain .................................. 77
   Markus U. Ehrengruber and Kenneth Lundstrom
8  Fluorescent Ligands and TR-FRET to Study Receptor-Receptor
   Interactions in the Brain ................................... 99
   Victor Fernández-Dueñas, Thierry Durroux, and Francisco
   Ciruela
9  In Situ Proximity Ligation Assay to Study and Understand
   the Distribution and Balance of GPCR Homo- and
   Heteroreceptor Complexes in the Brain ...................... 109
   Dasiel O. Borroto-Escuela, Beth Hagman, Miles Woolfenden,
   Luca Pinton, Antonio Jiménez-Beristain, Julia Oflijan,
   Manuel Narvaez, Michael Di Palma, Kristin Feltmann, 
   Stfano Sartini, Patrizia Ambrogini, Francisco Ciruela, 
   Riccardo Cuppini, and Kjell Fuxe

Part II. Neuroanatomical Techniques
10 Fluorescent In Situ Hybridization for Sensitive and 
   Specific Labeling .......................................... 127
   Miwako Yamasaki and Masahiko Watanabe
11 Autoradiographic Visualization of G Protein-Coupled 
   Receptors in Brain ......................................... 143
   Rebeca Vidal, Raquel Linge, Maria Josefa Castillo, Josep 
   Amigó, Elsa M. Valdizán, and Elena Castro
12 Analysis of the Expression Profile and Regional
   Distribution of Neurotransmitter Receptors and Ion
   Channels in the Central Nervous System Using Histoblots .... 157
   Elek Molnár
13 Immunohistochemistry for Ion Channels and Their 
   Interacting Molecules: Tips for Improving Antibody 
   Accessibility .............................................. 171
   Kohtarou Konno and Masahiko Watanabe
14 Localization of GFP-Tagged Proteins at the Electron 
   Microscope ................................................. 179
   Sara Gil-Perotin, A. Cebrián-Silla, V. Herranz-Pérez,
   P. García-Belda, S. Gil-García, M. Fil, J.S. Lee, 
   M.V. Nachury, and José Manuel García-Verdugo
15 Pre-embedding Methods for the Localization of Receptors 
   and Ion Channels ........................................... 191
   Rafael Luján
16 Post-embedding Immunohistochemistry in the Localisation
   of Receptors and Ion Channels .............................. 211
   Rafael Luján and Masahiko Watanabe
17 High-Resolution Localization of Membrane Proteins by
   SDS-Digested Freeze-Fracture Replica Labeling (SDS-FRL) .... 233
   Harumi Harada and Ryuichi Shigemoto
18 Application of Virus Vectors for Anterograde Tract-Tracing
   and Single-Neuron Labeling Studies ......................... 247
   Hiroyuki Hioki, Hisashi Nakamura, and Takahiro Furuta
19 Analysis of Synaptic Connections at the Electron 
   Microscopic Level Using Viral Vectors ...................... 267
   Takahiro Furuta, Keiko Okamoto-Furuta, and Hiroyuki Hioki
20 Morphological and Neurochemical Characterization of
   Electrophysiologically Identified Cells .................... 277
   Yoshiyuki Kubota

Part III. Functional Techniques
21 Using Electrophysiology to Study Synaptic and
   Extrasynaptic Ionotropic Receptors in Hippocampal Neurons .. 313
   Ian D. Coombs and David Soto
22 Biophysical Methods to Analyze Direct G-Protein 
   Regulation of Neuronal Voltage-Gated Calcium Channels ...... 357
   Norbert Weiss and Michel De Waard
23 Electrophysiological Recordings in Behaving Animals ........ 369
   Agnès Gruart and José M. Delgado-García
24 Voltammetry in Behaving Animals ............................ 397
   Kendra D. Bunner and George V. Rebec
25 In Vivo Brain Microdialysis of Monoamines .................. 415
   Jorge E. Ortega, J. Javier Meana, and Luis F. Callado
26 Calcium Transients in Single Dendrites and Spines
   of Pyramidal Neurons In Vitro .............................. 435
   Rbiannon M. Meredith and Martine R. Groen
27 Dynamic Recording of Membrane Potential from Hippocampal
   Neurons by Using a FRET-Based Voltage Biosensor ............ 447
   Victor Fernández-Dueñas, Xavier Moratу, Thomas Knbpfel, 
   and Francisco Ciruela
28 Determination of GPCR-Mediated cAMP Accumulation in Rat
   Striatal Synaptosomes ...................................... 455
   Jaume Taura, Victor Ferndnáez-Dueñas, and Francisco 
   Ciruela
29 GPCR-Mediated MAPK/ERK Cascade Activation in Mouse
   Striatal Slices ............................................ 465
   Maricel Gómez-Soler, Victor Fernández-Dueñas, and
   Francisco Ciruela

Subject Index ................................................. 473


Архив выставки новых поступлений | Отечественные поступления | Иностранные поступления | Сиглы
 

[О библиотеке | Академгородок | Новости | Выставки | Ресурсы | Библиография | Партнеры | ИнфоЛоция | Поиск]
  Пожелания и письма: branch@gpntbsib.ru
© 1997-2024 Отделение ГПНТБ СО РАН (Новосибирск)
Статистика доступов: архив | текущая статистика
 

Документ изменен: Wed Feb 27 14:29:52 2019. Размер: 9,992 bytes.
Посещение N 786 c 07.11.2017